International Journal of Medical Science and Public Health

2018, Vol: 7, Issue: 10

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Int J Med Sci Public Health. 2017; 6(5): 951-955

doi: 10.5455/ijmsph.2017.1267623012017

Detection of phospholipase production by EGG Yolk-Agar in Malassezia isolates from diseased and healthy human host

Charu Jain, Shukla Das, Rumpa Saha, V G Ramachandran, S N Bhattacharya, Sajad Dar.

Abstract
Background: Malassezia species (spp.) are cutaneous opportunistic pathogens and associated with various dermatological diseases. Virulence traits include a variety of enzymes such as lipases, phospholipase, and hyaluronidase. Phenotypic determination of phospholipase production in isolates from diseased group can confirm the pathogenic potential of the enzyme.

Objective: To find out the presence of phospholipases activity in the isolates of Malassezia species from three dermatologic conditions, i.e., atopic dermatitis, seborrheic dermatitis, and pityriasis versicolor; and comparative analysis with isolates from healthy host.

Materials and Methods: It was a prospective observational study conducted for a period 12-month to determination and quantify extracellular phospholipase activity (Pz) by the EGG Yolk-Agar method.

Results: Determination of extracellular Pz, by the EGG Yolk-Agar method was done on all the Malassezia isolates, for assessing its role as a potential virulence factor. Statistically significant results, for the phospholipase, were obtained in the patient group for Malassezia sympodialis.

Conclusions: The study demonstrated an association between pathogenic isolates and phospholipase production as compared to isolates from the healthy host. Such findings highlight the importance of phospholipase enzyme in virulence determination of a yeasts which is generally considered a commensal.

Key words: Malassezia sympodialis; Phospholipase; Extracellular; Dermatitis; Pityriasis Versicolor

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Pubmed Style

Jain C, Das S, Saha R, Ramachandran VG, Bhattacharya SN, Dar S. Detection of phospholipase production by EGG Yolk-Agar in Malassezia isolates from diseased and healthy human host. Int J Med Sci Public Health. 2017; 6(5): 951-955. doi:10.5455/ijmsph.2017.1267623012017

Web Style

Jain C, Das S, Saha R, Ramachandran VG, Bhattacharya SN, Dar S. Detection of phospholipase production by EGG Yolk-Agar in Malassezia isolates from diseased and healthy human host. http://www.ijmsph.com/?mno=251937 [Access: October 15, 2018]. doi:10.5455/ijmsph.2017.1267623012017

AMA (American Medical Association) Style

Jain C, Das S, Saha R, Ramachandran VG, Bhattacharya SN, Dar S. Detection of phospholipase production by EGG Yolk-Agar in Malassezia isolates from diseased and healthy human host. Int J Med Sci Public Health. 2017; 6(5): 951-955. doi:10.5455/ijmsph.2017.1267623012017

Vancouver/ICMJE Style

Jain C, Das S, Saha R, Ramachandran VG, Bhattacharya SN, Dar S. Detection of phospholipase production by EGG Yolk-Agar in Malassezia isolates from diseased and healthy human host. Int J Med Sci Public Health. (2017), [cited October 15, 2018]; 6(5): 951-955. doi:10.5455/ijmsph.2017.1267623012017

Harvard Style

Jain, C., Das, S., Saha, R., Ramachandran, V. G., Bhattacharya, S. N. & Dar, S. (2017) Detection of phospholipase production by EGG Yolk-Agar in Malassezia isolates from diseased and healthy human host. Int J Med Sci Public Health, 6 (5), 951-955. doi:10.5455/ijmsph.2017.1267623012017

Turabian Style

Jain, Charu, Shukla Das, Rumpa Saha, V G Ramachandran, S N Bhattacharya, and Sajad Dar. 2017. Detection of phospholipase production by EGG Yolk-Agar in Malassezia isolates from diseased and healthy human host. International Journal of Medical Science and Public Health, 6 (5), 951-955. doi:10.5455/ijmsph.2017.1267623012017

Chicago Style

Jain, Charu, Shukla Das, Rumpa Saha, V G Ramachandran, S N Bhattacharya, and Sajad Dar. "Detection of phospholipase production by EGG Yolk-Agar in Malassezia isolates from diseased and healthy human host." International Journal of Medical Science and Public Health 6 (2017), 951-955. doi:10.5455/ijmsph.2017.1267623012017

MLA (The Modern Language Association) Style

Jain, Charu, Shukla Das, Rumpa Saha, V G Ramachandran, S N Bhattacharya, and Sajad Dar. "Detection of phospholipase production by EGG Yolk-Agar in Malassezia isolates from diseased and healthy human host." International Journal of Medical Science and Public Health 6.5 (2017), 951-955. Print. doi:10.5455/ijmsph.2017.1267623012017

APA (American Psychological Association) Style

Jain, C., Das, S., Saha, R., Ramachandran, V. G., Bhattacharya, S. N. & Dar, S. (2017) Detection of phospholipase production by EGG Yolk-Agar in Malassezia isolates from diseased and healthy human host. International Journal of Medical Science and Public Health, 6 (5), 951-955. doi:10.5455/ijmsph.2017.1267623012017



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