E-ISSN 2277-338X | ISSN 2320-4664
 

Original Research 


Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37C

Nilam S Karbhari, Dipti S Karbhari, Manmeet Kochar, Shailesh Patel.

Abstract
Background: Laboratory analysis of alkaline phosphatase (ALP) from blood can detect abnormalities which reflect changes in certain health conditions, including liver disease and bone disease. Despite the importance of measuring different isoenzymes activity in different diseases, total ALP test is often requested, which is not specific to identify the type and the extent of tissue damage. ALP activity measurement at different incubation period may affect the actual measurement as ALP is a heat labile enzyme and specific ALP isoenzymes have different sensitivity to heat inactivation which creates lab to lab variability and significant change if particular isoenzyme is predominant in patients sera.

Objectives: The study was performed to measure the loss of activity of ALP at different 37C incubation periods during ALP measurement in serum.

Materials and Methods: Quality control materials were analyzed for ALP enzyme activity in 2-amino, 2-methyl, 1-propanol buffer, and 4-nitrophenyl phosphate substrate with different incubation periods.

Result: There is a gradual decrease in enzyme activity on increase of incubation period.

Conclusion: The effect of incubation period on ALP activity measurement at 37C may be clinically relevant if one of the isoenzymes is predominant in the patient sera.

Key words: Enzyme Measurement; Alkaline Phosphatase; Heat Inactivation; Incubation


 
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Pubmed Style

Karbhari NS, Karbhari DS, Kochar M, Patel S. Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37C. Int J Med Sci Public Health. 2017; 6(6): 1042-1045. doi:10.5455/ijmsph.2017.1270115022017


Web Style

Karbhari NS, Karbhari DS, Kochar M, Patel S. Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37C. http://www.ijmsph.com/?mno=254340 [Access: May 25, 2018]. doi:10.5455/ijmsph.2017.1270115022017


AMA (American Medical Association) Style

Karbhari NS, Karbhari DS, Kochar M, Patel S. Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37C. Int J Med Sci Public Health. 2017; 6(6): 1042-1045. doi:10.5455/ijmsph.2017.1270115022017



Vancouver/ICMJE Style

Karbhari NS, Karbhari DS, Kochar M, Patel S. Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37C. Int J Med Sci Public Health. (2017), [cited May 25, 2018]; 6(6): 1042-1045. doi:10.5455/ijmsph.2017.1270115022017



Harvard Style

Karbhari, N. S., Karbhari, D. S., Kochar, M. & Patel, S. (2017) Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37C. Int J Med Sci Public Health, 6 (6), 1042-1045. doi:10.5455/ijmsph.2017.1270115022017



Turabian Style

Karbhari, Nilam S, Dipti S Karbhari, Manmeet Kochar, and Shailesh Patel. 2017. Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37C. International Journal of Medical Science and Public Health, 6 (6), 1042-1045. doi:10.5455/ijmsph.2017.1270115022017



Chicago Style

Karbhari, Nilam S, Dipti S Karbhari, Manmeet Kochar, and Shailesh Patel. "Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37C." International Journal of Medical Science and Public Health 6 (2017), 1042-1045. doi:10.5455/ijmsph.2017.1270115022017



MLA (The Modern Language Association) Style

Karbhari, Nilam S, Dipti S Karbhari, Manmeet Kochar, and Shailesh Patel. "Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37C." International Journal of Medical Science and Public Health 6.6 (2017), 1042-1045. Print. doi:10.5455/ijmsph.2017.1270115022017



APA (American Psychological Association) Style

Karbhari, N. S., Karbhari, D. S., Kochar, M. & Patel, S. (2017) Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37C. International Journal of Medical Science and Public Health, 6 (6), 1042-1045. doi:10.5455/ijmsph.2017.1270115022017





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