E-ISSN 2277-338X | ISSN 2320-4664
 

Original Research 


Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC

Abhishek Kumar Jain, Savita Bharat Jain, K P Ranjan, Vaibhav Misra, Shashi Gandhi.

Abstract
Background: Throughout the world multidrug-resistant healthcare-associated infections (HAI) are one of the leading causes of morbidity and mortality among hospitalized patients, leading to a major burden on public health system of any country. An intensive care unit (ICU) patient has five- to seven-folds higher risk of HAI and ICU infections contribute to 2025% of all HAI.

Objective: This prospective study was designed to isolate and identify the bacterial etiology, their antimicrobial susceptibility pattern and to detect the production of the metallo β-lactamases (MBL) and AmpC-β-lactamases in multidrug-resistant gram negative isolates.

Material and Methods: The study was conducted for the period of 1 year. During the period a total 196 samples were collected and processed as per Clinical and Laboratory Standards Institute guidelines.

Result: The majority of bacterial isolates causing HAI were found to be Gram-negative bacilli (73.68%). Acinetobacter species followed by Pseudomonas aeruginosa, Escherichia coli, Citrobacter species, Klebsiella species, and Enterobacter species, respectively. Gram-positive cocci were accounted 26.31% isolates. All isolates were multidrugresistant. Among Gram negative bacilli, 38.10% AmpC producer, 35.71% MBL, and 16.67% were coproducer of AmpC and MBL.

Conclusion: Microbiological profile of infection in ICU of our institute was multidrug-resistant, and in many isolates, drug resistance was due to the production of MBL and AmpC-β-lactamases, which represents basic information for future monitoring of HAI and could be repeated periodically. Thus, the future prevention program should focus on patients with a longer length of stay and those with invasive devices.

Key words: Healthcare-Associated Infections; metallo β-lactamases; AmpC-β-lactamases; ICU


 
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Pubmed Style

Abhishek Kumar Jain, Savita Bharat Jain, K P Ranjan, Vaibhav Misra, Shashi Gandhi. Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC. Int J Med Sci Public Health. 2018; 7(3): 229-234. doi:10.5455/ijmsph.2018.1027919012018


Web Style

Abhishek Kumar Jain, Savita Bharat Jain, K P Ranjan, Vaibhav Misra, Shashi Gandhi. Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC. http://www.ijmsph.com/?mno=279909 [Access: November 21, 2018]. doi:10.5455/ijmsph.2018.1027919012018


AMA (American Medical Association) Style

Abhishek Kumar Jain, Savita Bharat Jain, K P Ranjan, Vaibhav Misra, Shashi Gandhi. Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC. Int J Med Sci Public Health. 2018; 7(3): 229-234. doi:10.5455/ijmsph.2018.1027919012018



Vancouver/ICMJE Style

Abhishek Kumar Jain, Savita Bharat Jain, K P Ranjan, Vaibhav Misra, Shashi Gandhi. Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC. Int J Med Sci Public Health. (2018), [cited November 21, 2018]; 7(3): 229-234. doi:10.5455/ijmsph.2018.1027919012018



Harvard Style

Abhishek Kumar Jain, Savita Bharat Jain, K P Ranjan, Vaibhav Misra, Shashi Gandhi (2018) Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC. Int J Med Sci Public Health, 7 (3), 229-234. doi:10.5455/ijmsph.2018.1027919012018



Turabian Style

Abhishek Kumar Jain, Savita Bharat Jain, K P Ranjan, Vaibhav Misra, Shashi Gandhi. 2018. Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC. International Journal of Medical Science and Public Health, 7 (3), 229-234. doi:10.5455/ijmsph.2018.1027919012018



Chicago Style

Abhishek Kumar Jain, Savita Bharat Jain, K P Ranjan, Vaibhav Misra, Shashi Gandhi. "Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC." International Journal of Medical Science and Public Health 7 (2018), 229-234. doi:10.5455/ijmsph.2018.1027919012018



MLA (The Modern Language Association) Style

Abhishek Kumar Jain, Savita Bharat Jain, K P Ranjan, Vaibhav Misra, Shashi Gandhi. "Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC." International Journal of Medical Science and Public Health 7.3 (2018), 229-234. Print. doi:10.5455/ijmsph.2018.1027919012018



APA (American Psychological Association) Style

Abhishek Kumar Jain, Savita Bharat Jain, K P Ranjan, Vaibhav Misra, Shashi Gandhi (2018) Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC. International Journal of Medical Science and Public Health, 7 (3), 229-234. doi:10.5455/ijmsph.2018.1027919012018





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