E-ISSN 2277-338X | ISSN 2320-4664
 

Original Research 


Chikungunya fever, a reemerging virus infection: Diagnosed by real-time polymerase chain reaction

Nidhi Barot, Umang Patel, Viral Pathak, Nruta Mehta, Priyanka Thaker.

Abstract
Background: Chikungunya virus (CHIKV), an emerging viral infection, has become a serious health problem in recent years. It has been frequently reported in the Indian subcontinent in the past few years. Reemergence of the other arboviral hemorrhagic fever has made diagnostic dilemma for clinicians. The prognosis of the disease is generally good; though some patients develop chronic arthritis and neurological involvement have been reported.

Objective: To study the epidemiological and clinical profile of CHIKV infection.

Materials and Methods: Study comprises total 1296 patients presented with clinical suspicion of Chikungunya from September to December 2016. Confirmation of cases was carried out by detection of viral RNA by real-time polymerase chain reaction (RT-PCR).

Result: Of 1296 suspected cases, 845 (65.2%) cases were positive for CHIKV RNA, detected by RT-PCR assay. Majority of the confirmed cases were of age group 5059 years. Male:female positivity ratio was 1.33:1. The most common clinical features were fever with joint pain and rash. Of 34 suspected cases with neurological involvement, 14 cases (41.17%) were confirmed positive by RT-PCR in cerebrospinal fluid specimen.

Conclusion: Early diagnosis and monitoring of CHIKV infection is an important component of disease management. Viral RNA detection by RT-PCR gives a positive confirmatory result in the acute phase (first 7 days) of the disease. RT-PCR is rapid, specific, and sensitive method of choice for the early detection and confirmation of virus in clinical samples.

Key words: Chikungunya Virus Infection; Viral RNA detection; Real-time Polymerase Chain Reaction, Rapid, Specific, Sensitive


 
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Pubmed Style

Barot N, Patel U, Pathak V, Mehta N, Thaker P. Chikungunya fever, a reemerging virus infection: Diagnosed by real-time polymerase chain reaction. Int J Med Sci Public Health. 2018; 7(4): 287-290. doi:10.5455/ijmsph.2018.0103801022018


Web Style

Barot N, Patel U, Pathak V, Mehta N, Thaker P. Chikungunya fever, a reemerging virus infection: Diagnosed by real-time polymerase chain reaction. http://www.ijmsph.com/?mno=289692 [Access: April 21, 2018]. doi:10.5455/ijmsph.2018.0103801022018


AMA (American Medical Association) Style

Barot N, Patel U, Pathak V, Mehta N, Thaker P. Chikungunya fever, a reemerging virus infection: Diagnosed by real-time polymerase chain reaction. Int J Med Sci Public Health. 2018; 7(4): 287-290. doi:10.5455/ijmsph.2018.0103801022018



Vancouver/ICMJE Style

Barot N, Patel U, Pathak V, Mehta N, Thaker P. Chikungunya fever, a reemerging virus infection: Diagnosed by real-time polymerase chain reaction. Int J Med Sci Public Health. (2018), [cited April 21, 2018]; 7(4): 287-290. doi:10.5455/ijmsph.2018.0103801022018



Harvard Style

Barot, N., Patel, U., Pathak, V., Mehta, N. & Thaker, P. (2018) Chikungunya fever, a reemerging virus infection: Diagnosed by real-time polymerase chain reaction. Int J Med Sci Public Health, 7 (4), 287-290. doi:10.5455/ijmsph.2018.0103801022018



Turabian Style

Barot, Nidhi, Umang Patel, Viral Pathak, Nruta Mehta, and Priyanka Thaker. 2018. Chikungunya fever, a reemerging virus infection: Diagnosed by real-time polymerase chain reaction. International Journal of Medical Science and Public Health, 7 (4), 287-290. doi:10.5455/ijmsph.2018.0103801022018



Chicago Style

Barot, Nidhi, Umang Patel, Viral Pathak, Nruta Mehta, and Priyanka Thaker. "Chikungunya fever, a reemerging virus infection: Diagnosed by real-time polymerase chain reaction." International Journal of Medical Science and Public Health 7 (2018), 287-290. doi:10.5455/ijmsph.2018.0103801022018



MLA (The Modern Language Association) Style

Barot, Nidhi, Umang Patel, Viral Pathak, Nruta Mehta, and Priyanka Thaker. "Chikungunya fever, a reemerging virus infection: Diagnosed by real-time polymerase chain reaction." International Journal of Medical Science and Public Health 7.4 (2018), 287-290. Print. doi:10.5455/ijmsph.2018.0103801022018



APA (American Psychological Association) Style

Barot, N., Patel, U., Pathak, V., Mehta, N. & Thaker, P. (2018) Chikungunya fever, a reemerging virus infection: Diagnosed by real-time polymerase chain reaction. International Journal of Medical Science and Public Health, 7 (4), 287-290. doi:10.5455/ijmsph.2018.0103801022018





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