E-ISSN 2277-338X | ISSN 2320-4664
Required files to uploaded
|Original Research|| |
Estimation of lymphocyte apoptosis in patients with chronic, non healing diabetic foot ulcer
Awadhesh Kumar Arya, Sunny Garg, Santosh Kumar, Lalit P Meena, Kamlakar Tripathi.
|Cited by (1)|
Background: Lymphocytes play an important role in wound healing and the removal of circulating T lymphocytes inhibits the healing cascade. Decreased stimulation of survival factors and increased levels of dead signals may lead to the malfunction of many cells, including lymphocytes.
Aims & Objective: To explore lymphocytes involvement in wound healing.
Material and Methods: Study participants were divided into three groups: group A, group B and group C (30 participants each). Annexin-V-FITC+CD-3-PE kit were used for the lymphocyte apoptosis estimation in diabetic foot patients by florescent activated cell sorter (FACS).
Results: We find out significantly higher total T cell apoptosis in type 2 diabetes mellitus patients having chronic, non healing diabetic foot ulcer as compared with healthy individuals. CD-3 + Annexin-V-FITC positive lymphocytes were statistically significant in group C (P<0.01) and group B (P<0.001) when compared from group A.
Conclusion: This study suggests the importance of T-lymphocytes in wound healing. From the present study we can suggest diabetic patients to maintain their immune system for the normal wound healing.
Diabetic foot, Apoptosis, Lymphocyte
|1. American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus. Diabetes care. 2010;33:S62-69. [DOI via Crossref] |
|2. Guo S, DiPietro LA. Factors Affecting Wound Healing. J Dent Res. 2010; 89(3): 219-229. [DOI via Crossref] |
|3. Schaffer M, Barbul A. Lymphocyte function in wound healing and following injury. Br J Surg. 1998; 85:444-460. [DOI via Crossref] |
|4. Wahl SM, Allen JB. T lymphocyte-dependent mechanisms of fibrosis. Prog Clin Biol Res. 1988; 266:147-60.|
|5. Blotnik S, Peoples GE, Freeman MR, Eberlein TJ, Klagsbrun M. T lymphocytes synthesize and export heparin-binding epidermal growth factor-like growth factor and basic fibroblast growth factor, mitogens for vascular cells and fibroblasts: differential production and release by CD41 and CD81 T cells. Proc Natl Acad Sci. 1994; 91: 2890-94. [DOI via Crossref] |
|6. Swift ME, Burns AL, Gray KL, DiPietro LA. Age-related alterations in the inflammatory response to dermal injury. J Invest Dermatol. 2001;117:1027-35. [DOI via Crossref] |
|7. Schaffer M, Stulten C, Viebahn R. Role of T-lymphocytes in radiation-impaired wound healing. Pol Przegl Chir. 2007;79(4):312-18. [DOI via Crossref] |
|8. Efron JE, Frankel HL, Lazarou SA, Wasserkrug HL, Barbul A. Wound Healing and T-Lymphocytes. J Surg Res. 1990;48:460-63. [DOI via Crossref] |
|9. Arya AK, Pokharia D, Tripathi K. Relationship between oxidative stress and apoptotic markers in lymphocytes of diabetics with chronic non healing wound. Diabetes Res Clin Pract. 2011, 94(3):377-384. [DOI via Crossref] |
|10. Neilson EG, Phillips SM, Jimenez S. Lymphokine moduation of fibroblast proliferation. J Immunol. 1982; 128:1484-86.|