E-ISSN 2277-338X | ISSN 2320-4664
 

Original Research 


Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis

Bipin Kumar Singh, Kirti Singh, Rahul Khanna, Ram Niwas Meena.

Abstract
Background: It is still difficult to diagnose tuberculosis as a source of abdominal pain. Due to the lack of non-invasive diagnostic testing, the diagnosis remains a problem. Determining the role of polymerase chain reaction (PCR) in ascitic fluid in the diagnosis of abdominal tuberculosis, we can conclude whether it is sensitive/specific or not and further its use in the diagnosis of abdominal tuberculosis.

Objective: The objective of the study was to determine the role of PCR in ascitic fluid in the diagnosis of abdominal tuberculosis and also to correlate the ADA levels of ascitic fluid with the PCR report.

Materials and Methods: This prospective comparative study includes 41 clinically suspected abdominal tuberculosis patients (age range 1565 years) over a period of 2 years. A detailed history, clinical evaluation, and relevant investigations, including radiology, were done in all patients. Bacteriological or histological approaches had to support the diagnosis of abdominal tuberculosis, and PCR was also tested for Mycobacterium tuberculosis in ascitic fluid.

Results: The mean age of patients was 31.7112.32 years with male:female ratio was 1.4:1. PCR was positive in 24 (58.8%) cases. A receiver operating characteristic curve showed that a cutoff value of 35.3 IU/L (AUC 0.998, P < 0.001) for the ADA level produced the best results as a diagnostic test for abdominal tuberculosis, yielding the following parameter values: Sensitivity 100%, specificity 94.1%, positive predictive value 96%, negative predictive value 100%, and diagnostic accuracy 97.56%. ADA values were significantly elevated during abdominal tuberculosis, indicating that ADA can still be a valuable diagnostic tool.

Conclusion: Our findings indicate that ascitic fluid PCR is a safe tool for diagnosing it and should be tried at least before surgical intervention.

Key words: Abdominal Tuberculosis; Polymerase Chain Reaction; Ascitic Fluid; Adenosine Deaminase


 
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How to Cite this Article
Pubmed Style

Singh BK, Singh K, Khanna R, Meena RN. Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis. Int J Med Sci Public Health. 2020; 9(6): 368-374. doi:10.5455/ijmsph.2020.12343201930062020


Web Style

Singh BK, Singh K, Khanna R, Meena RN. Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis. http://www.ijmsph.com/?mno=76843 [Access: August 29, 2020]. doi:10.5455/ijmsph.2020.12343201930062020


AMA (American Medical Association) Style

Singh BK, Singh K, Khanna R, Meena RN. Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis. Int J Med Sci Public Health. 2020; 9(6): 368-374. doi:10.5455/ijmsph.2020.12343201930062020



Vancouver/ICMJE Style

Singh BK, Singh K, Khanna R, Meena RN. Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis. Int J Med Sci Public Health. (2020), [cited August 29, 2020]; 9(6): 368-374. doi:10.5455/ijmsph.2020.12343201930062020



Harvard Style

Singh, B. K., Singh, . K., Khanna, . R. & Meena, . R. N. (2020) Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis. Int J Med Sci Public Health, 9 (6), 368-374. doi:10.5455/ijmsph.2020.12343201930062020



Turabian Style

Singh, Bipin Kumar, Kirti Singh, Rahul Khanna, and Ram Niwas Meena. 2020. Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis. International Journal of Medical Science and Public Health, 9 (6), 368-374. doi:10.5455/ijmsph.2020.12343201930062020



Chicago Style

Singh, Bipin Kumar, Kirti Singh, Rahul Khanna, and Ram Niwas Meena. "Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis." International Journal of Medical Science and Public Health 9 (2020), 368-374. doi:10.5455/ijmsph.2020.12343201930062020



MLA (The Modern Language Association) Style

Singh, Bipin Kumar, Kirti Singh, Rahul Khanna, and Ram Niwas Meena. "Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis." International Journal of Medical Science and Public Health 9.6 (2020), 368-374. Print. doi:10.5455/ijmsph.2020.12343201930062020



APA (American Psychological Association) Style

Singh, B. K., Singh, . K., Khanna, . R. & Meena, . R. N. (2020) Role of polymerase chain reaction of ascitic fluid and its correlation with adenosine deaminase levels in the diagnosis of abdominal tuberculosis. International Journal of Medical Science and Public Health, 9 (6), 368-374. doi:10.5455/ijmsph.2020.12343201930062020





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